Associations between Homocysteine Levels and Oxidative Stress Biomarkers in Placental of Preeclampsia Iraqi Patients

Hassan F. Al-Azzawie, Esraa H. Humadi


Objective: Oxidative stress occurs when cellular levels of reactive oxygen species exceed antioxidant
capabilities and has been implicated in the pathogenesis of preeclampsia.
Patients and Methods: In this study we measured the tissue levels of endogenous antioxidant
proteins such as superoxide dismutase, glutathione peroxidase, glutathione reductase and in addition
to the levels of homocysteine, lipid and protein oxidation in placental samples from normal and
preeclamptic pregnancies.
Results: Preeclamptic tissue homogenates demonstrated significantly increased levels of
homocysteine 12.31 ± 2.10 versus 2.31 ± 3.22 μmole/l, increased levels of lipid peroxidation 23.77 ±
5.26 μM/ mg protein versus 6.22 ± 2.31 μM/ mg protein (p< 0.01) and a significance increase in
protein carbonyl concentration 250.44 ± 48.23 versus 190.24 ± 26 .46 units/mg protein when
compared to controls, while levels of the other antioxidant proteins, superoxide dismutase1.55 ± 0.22
versus4.42 ± 0.32 U/mg protein p<0.05, Glutathionereductase12.13+ 3.3 versus 22.24 ± 7.71 U/mg
protein (P<0.05), Glutathione68.12 ± 14.22 versus 105 ± 16ng/mg protein (P<0.01) and glutathione
peroxidase 10. 22 ± 5.33 versus15.22 ± 4.44 nmol/min /mg protein (P<0.05) were all found to be
significantly reduced when comparing preeclamptic placental tissue homogenates to gestational age
matched control placental from non preeclamptic pregnancies.
Conclusions: The results of this study demonstrate placental oxidative stress through reduced
antioxidant enzyme activities and hyperhomocystinemia which play a role in the pathogenesis of
preeclampsia .These novel data further understanding the pathophysilology of preeclampsia and
provides a new insight into the pathogenesis of clinical complications exhibited in this condition
suggesting antioxidant therapy may be improving health of this condition.


Homocysteine, Oxidative Stress Markers, Preeclampsia Placental

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