The Histological Changes of Albino Mice Embryos' Kidneys after Exposure In Utero to Topiramate

Fatin Th. Abdul-Rhahman, Luma I. Al-Allaf, Hatim A. Al-Nuaimy



Background: The kidneys can be damaged by a large number of therapeutic agents. Mechanisms of injury are varied and all renal structures may be affected.

Objectives: To discuss the effect of topiramate (one of the new antiepileptic drugs) on renal histology by studying the pathological features of the kidneys of embryos of albino mice, Balbls species (Swiss origin, Mus musculus) after intrauterine exposure to that drug and to determine the frequencies of these pathological findings.

Setting: Laboratory of Postgraduate Studies at the Department of Anatomy, Histology and Embryology in Mosul College of Medicine, Mosul University, Mosul, Northern Iraq.

Methods: This study was conducted over a period of 2 months extending from July 1, 2010 to September 1, 2010. Male and female albino mice, Balbls species (Swiss origin, Mus musculus) were chosen from 10-12 weeks of age, their mean weight was about 28±2 gms and they were healthy. These animals were put in good environmental conditions and underwent acclimatization in a 12 hour light-dark cycle. The animals were put in plastic cages with food and water ad libitum.

Animals with age of 10-14 weeks and weight of 25 gms from both sexes were chosen for mating and the insurance of mating was made by noticing the vaginal plug .The pregnant females were isolated with the recording of the date of mating as day zero. Each pregnant female was housed in single cage with good environmental conditions for the duration of pregnancy and lactation and after weaning the males were isolated from females in big plastic cages with food and water ad libitum. Seventy five pregnant mices were used in this study and they were classified in two groups: Group One: consisted of 30 pregnant females with distilled water administration (Control Group). Group Two: consisted of 45 pregnant females with topiramate oral administration of 8.4 mg /Kg body weight (Study Group).The oral administration was done daily from day one of pregnancy for eighteen days.

The pregnant females from both groups were dissected using Ether inhalations at day eighteen of pregnancy .The pregnant females were dissected and the embryos were obtained from their uteruses and they were immersed for a few seconds in Nacl solution 0.9% to get rid of superficial blood. The embryos were examined using a magnifying lens .The weight of all embryos were measured using a sensitive electronic measure.

Two slices of 3-4 mm thickness from both kidneys were then taken and run in staining protocols using alcoholic Bouin’s fixative, and then transferred to the Laboratory of Postgraduate Studies at the

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