Amlodipine Inhibits Cell Proliferation and Induces Cell Cycle Arrest in Colorectal Cancer Cells

Mohammad A Y Alqudah, Baraah A Alrababah, Nizar Mahmoud Mhaidat


Amlodipine, a dihydropyridine Ca2+ channel blocker, was previously shown to exhibits antitumor effects on
different human cancer cells both in vitro and in vivo, through inhibition of Ca2+ cell entry. However, up to our
knowledge, amlodipine antitumor effect has not been examined before on colorectal cancer cells (CRC). In this
study, the effect of amlodipine on cell proliferation, cell cycle and apoptosis was examined on CRC cells. In two
different cell lines, treatment of CRC cells with 50 uM amlodipine resulted in significant reduction of cell viability
compared to DMSO-treated cells (IC50 values were 27.17 μM for HCT116 cells and 37.69 μM for SW480 cells).
Flow cytometric analysis using propidium iodide revealed that treatment with amlodipine (50 uM, for 48 h)
induced G1 phase cell accumulation compared to DMSO-treated cells in both cell lines. However, treatment with
amlodipine (50 uM for 48 h) did not induce cellular apoptosis in CRC cells. Our finding showed that amlodipine
has significant antiproliferative effect on CRC cells, where G1 cell cycle arrest is partially responsible for this
growth inhibitory action of amlodipine.


Amlodipine; colorectal cancer; cell cycle; apoptosis; proliferation

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