Evaluation of in vitro salt tolerance in Cucumis prophetarum L., a Crop Wild Relative

Wesam Al Khateeb


Salinity is the major abiotic factor that limits plant growth and productivity. In this study, we present an in vitro propagation protocol for Cucumis prophetarum L. a crop wild relative to cucumber and compare the effect of salinity on C. sativus and C. prophetarum. Results showed that Murashige and Skoog (MS) medium supplemented with 1 mg L-1 Kin and 0.5 mg L-1 NAA was the optimal medium for shoot proliferation and root induction, respectively. Interestingly, C. prophetarum plants showed better growth responses than C. sativus under salinity stress. Furthermore, increased salinity level decreased lipid peroxidation content and increased proline content in C. prophetarum, but C. sativus showed opposite responses. In addition, C. sativus microshoots accumulate more Na than C. prophetarum. In conclusion, this study showed that C. prophetarum is more salt tolerant than C. sativus, this could be used in further work to improve salt tolerance traits in cucumber.


Cucumis prophetarum L., Cucumis sativus L., Crop wild relative, in vitro, micropropagation.

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