Isolation and Identification of Pseudomonas viridiflava, the causal agent of fruit rotting of Cucumis sativus

Nehaya Al-Karablieh, Ihsan Mutlak, Amani Al-Dokh

Abstract


Fruit rotting causes severe losses in cucumber crop grown in green houses in Jordan, rotting symptom is a common symptom for many bacterial infections. Therefore, the aim of this study was to precisely identify the bacterial causal agent which can be regarded as emergent causal agent in Jordan. Infected cucumber fruits with rotting symptom were sampled from different regions in Jordan, the samples were used in bacterial isolation and screening for putative plant pathogenic bacteria by LOPAT determinative tests. Nine representative Isolates were subjected to pathogenicity test, biochemical characterization and Identification by 16S-rDNA sequencing and in silico digestion by SacI and HinfI restriction enzymes. The isolates were characterized by production of yellowish mucoid materials on Mannitol-Glutamate (MG) medium, reproduce similar rotting symptoms on cucumber fruits as the field infection, typical LOPAT profile and similar biochemical profile to P. viridiflava, occurrence of seven sites for HinfI and absence of SacI site in the16S-rDNA and -based on 16S-rDNA sequences- it had been clustered with P. viridiflava stains. Therefore, it can be concluded that the causal agent of cucumber fruit rotting in Jordan was P. viridiflava.

Keywords


P. viridiflava, fruit rotting, LOPAT, 16S-rDNA

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