Ultrasonic Waves Stimulate the Activity of Thymine Nucleotide Biosynthetic Enzymes, Nucleic Acids and Proteins Content of Sesamum Indicum L. Stem Calli

Nihal E. Al-Taee, Sajida A. Abood, Mozahim K. Al-Mallah

Abstract


The present study revealed that the effect of ultrasonic waves in stimulating the activity of the enzymes thymidylate synthase (TS), dihydrofolate reductase (DHFR) and serine hydroxy methyl transeferase (SHMT), the amount of nucleic acids (DNA and RNA) and the protein content. In addition, the changes in fresh weight of seedling stem-derived callus of Sesamum indicum L. grown in initiation medium Murashige and Skoog (MS) + naphthalene acetic acid (NAA 1.0 mg L-1) + benzyl adenine (BA 2.0 mg L-1) which was previously exposed to 50 KHz of different periods (5, 10, 15, 20, 30, 40, 60 and 70 min). The period 40 min exposure had sustainable effect through enhancement the specific activity of TS, DHFR, and SHMT enzymes which recorded 2.08, 0.95 and 0.28 µmol/min/mg protein respectively compared with 1.48, 0.41 and 0.13 in control. The amounts of DNA and RNA were increased from 56, 530 to 75, 710 µg/g callus fresh weight respectively compared to control. On the other hand, the protein content and the fresh weight of callus were increased from 1.43 to 2.25 mg/g and 3.45 to 10.88 g respectively compared to control. Unfortunately the exposure period 70 min decreased the specific activity of the above enzymes, nucleic acids, as well as the fresh weight of callus.

Keywords


Thymine Nucleotide Biosynthetic Enzymes, Ultrasonic Waves, Callus, Sesame

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