The Effect of Local Injection of Mesenchymal Stem Cells and IL-10 with Platelet Lysate on Fracture Healing in a Rat Model

Khaldoon Mohammed Kair Bashaireh

Abstract


Objective: This study investigates the effect of mesenchymal stem cells (MSC), an immune-privileged cell source for tissue repair, Interluken (IL-10), an immune inhibitory factor, and platelet lysate (PL) on the fracture healing process.

Methods: Forty adult male Sprague-Dawley rats were anesthetized, and left tibia mid shaft fractures were created. The rats were randomly assigned to 5 groups and received injections with saline ( A); MSC and PL (B); IL-10 and PL (C); PL (D); and MSC, PL, and IL-10 (E). Lateral radiographs of four randomly selected rats in each group were taken at different days post-fracture. Four rats from each group were sacrificed at day twenty one post-fracture, with left tibiae harvested for histological analysis.

Results: At 7 days post-fracture, the highest level of callus formation and bone healing was observed in group E (treated with MSC, PL, and IL-10) followed by groups B (treated with MSC and PL). The same results were obtained at days 14, 21 and 28 post-fracture. At 21days post-fracture, histological analysis revealed that the callus of fractured tibiae in group A consisted mostly of chondrocytes, while in groups C and D, extensive remodeling with a limited amount of chondrocytes present at the fracture. Combination of MSC, PL, and IL-10was associated with the greatest fracture healing performance. After 3 weeks of treatment in group E and B, trabecular bone formation and cortex remodeling were observed.

Conclusions: The use of MSC in combination with IL-10 and PL is a promising alternative treatment to promote fracture healing.

Keywords


Bone regeneration, Mesenchymal stem cells, IL-10, Platelet lysate

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