Identification of Molecular Subgrouping of Medulloblastoma in Egyptian Patients: An immunohistochemical Study

Samaka RM, Abd El-Wahed MM, Kandil MA, Abd Elzaher AE, Shaaban IM, Abd Allah R

Abstract


To apply the immunohistochemical method using robust biomarkers; Gli1 & β catenin trying to identify sonic hedgehog (SHH) & Wingless (WNT) active tumors. Also identification of the molecular subgroups of medulloblastoma has immediate clinical relevance guiding therapeutic stratification and the use of targeted therapies.
This retrospective study was conducted on 49 tissue specimens of medulloblastoma cases for evaluation of immunohistochemical (IHC) expression of GLi1 and β-catenin.
The studied cases showed absence of nuclear positivity of β catenin & 15/49 cases (30.6%) exhibited an activated sonic hedgehog signaling pathway (SHH) pronounced by nuclear positivity for Gli1. 34/49 cases (69.4%) cases that showed absence of nuclear reactivity for Gli1 and β catenin were considered as non SHH/WNT medulloblastoma group. There were no statistical significant differences between the 2 subgroups as regards to the clinical and histopathological features.
Non SHH/WNT pathway identified via the absence of nuclear localization to either Gli1 & β catenin represents a major contributor to medulloblastoma in the studied Egyptian patients.

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