Effect of Mercuric Chloride on Glutathione (GSH) level in Plasma and Cytosolic Fraction of Human blood

M Farid Khan, Haroon Khan, Hashmatullah Khan, Rehan Z Paracha, Gul Majid Khan

Abstract


Elemental Mercury is the main ingredient in dental amalgams and in plasma-derived medicinal products and in other many medicines, but mercury is a neurotoxin and can harm human health if exposed. Thus, it is interesting to study the effect of mercurric chloride on the glutathione (GSH). The effect of mercuric chloride on the chemical status of the glutathione (GSH) in the whole blood has been studied using U.V Spectrophotometer by using Ellman’s method. The effect of mercurric chloride on the chemical status of glutathione (GSH) was checked in whole blood with the passage of time. It was found that there is a profound effect of decreasing the concentration of reduced glutathione (GSH) in whole blood, as the time has passed. The decrease in the glutathione level was dependent on concentration and time of interaction dependent, probably, due to oxidation of glutathione (GSH) to corresponding disulphide (GSSG). In this paper, the effect of mercuric chloride on thiol/GSH level was discussed in vitro, which in principal may present a model of in vivo reaction. This study can also help in a way to achieve the idea that the acute toxicity of the metal can be neutralized by the reduced glutathione (GSH).

Keywords


Mercury (Hg), Reduced Glutathione (GSH), Oxidize Glutathione (GSSG), Whole blood, Ellman’s Method, 5, 5-Dithiobis, 2-Nitrobenzoic Acid (DTNB)

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