Evaluation of Colchicine Effect on Cytokine Production from Peripheral Blood Mononuclear Cells of Type I Diabetes

Nizar Mhaidat, Karem Alzoubi, Omar Khabour, Maha Alomari, Ahmad Matalkah

Abstract


Problem: Type 1 diabetes mellitus (DM) results from autoimmune destruction of insulin-producing β cells and is characterized by the presence of insulitis and β-cell autoantibodies. Cytokines are known to play an important role in autoimmunity and have been suggested to be involved in the pathogenesis of insulin-dependent diabetes (IDDM). The aim of the present study was to evaluate the levels of major cytokines such as IL-2, IL-6, and IL-10 in Type I DM and in apparently healthy volunteers (control) and to study the effect of colchicine on the capacity of peripheral blood monocytes isolated from Type I diabetes patients and healthy volunteers with or without immune stimulation to produce IL-2, IL-6, and IL-10. Experimental approach: Fifty type I diabetes patients were randomly recruited from the Diabetes/Endocrine Clinics along with 15 healthy subjects. Levels of cytokines were measured from cultured blood monocytes using standard ELISA kits. Major findings: results revealed that basal levels of IL-2, IL-6, and IL-10 were significantly higher in diabetes group compared to healthy group (P < 0.05). LPS or PHA-induced production of these cytokines was significantly higher in diabetes group (P < 0.05). Moreover, pretreatment of stimulated monocytes of DM patients with colchicine inhibited cytokine levels to be comparable to that of cell cultured from healthy individuals. Conclusions: These results provide a new perspective on the protective role of colchicine against inflammatory long-term complications associated with type I DM.

Keywords


IDDM, IL-2; IL-6; IL-10, Pro-Inflammatory Cytokines, Anti- Inflammatory Cytokines

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