Simultaneous Spectrophotometric Determination of Tramadol and Acetaminophen in Pharmaceutical Formulations Using H-Point Standard Addition Method

Ali Reza Zarei


H-point standard addition method (HPSAM) was applied to the simultaneous spectrophotometric determination of tramadol and acetaminophen in pharmaceutical formulations. The results showed that tramadol and acetaminophen can be determined simultaneously in the range 5.0-100 and 1.0-10 µg/ml, respectively. The limit of detection (LOD) for tramadol and acetaminophen were obtained 2.0 and 0.50 µg/ml, respectively. Relative standard deviation for five replicate simultaneous determinations of tramadol and acetaminophen were calculated to be 3.2 and 3.3 %, respectively. The proposed method has been successfully applied to the simultaneous determination of tramadol and acetaminophen in some synthetic, pharmaceutical formulation.


Tramadol, Acetaminophen, Spectrophotometric, H-point standard addition method, Pharmaceutical formulation.


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