In vitro Modulation of Pancreatic MIN6 Insulin Secretion and Proliferation and Extrapancreatic Glucose Absorption by Paronychia argentea, Rheum ribes and Teucrium polium Extracts

Violet Kasabri, Rana Abu-Dahab, Fatma U. Afifi, Randa Naffa, Lara Majdalawi, Hazar Shawash

Abstract


The present study design investigated the effects of crude aqueous extracts (AE) of Paronychia argentea Lam., Rheum ribes L. and Teucrium polium L., traditionally utilized in diabetes treatment in Jordan, on the pancreatic β-cell MIN6 proliferation and insulin secretion and extrapancreatic glucose diffusion. In an ascending order, R. ribes, T. polium and P. argentea concentrations induced a MIN6 monolayers expansion by respective 118-136%, 158-175% and 140-200% (P<0.001), thus exceeding GLP-1 (5 nM) pancreatic proliferative capacity. Like L-alanine (10 mM) insulinotropic efficacy and without exerting cytotoxicity, glucose stimulated insulin secretion was potentiated by AEs of R. ribes (373-736%, P<0.001) and T. polium (503-1190% P<0.001). P. argentea AE was inactive at used doses. The potent plants’ insulin secretory bioactivities were abolished in the depleted Ca2+ conditions (P<0.001). Comparable to guar gum (50 mg/ml) diffusional hindrance in a simple dialysis model, P. argentea inhibited overnight glucose movement in vitro (by 38.1±1.9% AUC reductions, P<0.001), while R. ribes and T. polium AEs proved inactive. This in vitro evaluation has revealed that all three plants augmented β-cell expansion, P. argentea inhibited carbohydrate absorption and R. ribes and T. polium stimulated insulin secretion. These actions depend on their intact absorption in vivo. Future directives may assess the use of P. argentea, R. ribes and T. polium as new potential sources with functional properties for food or nutraceutical products or active leads into anti-diabetes pharmacotherapy.

Keywords


Paronychia argentea Lam, Rheum ribes L., Teucrium polium L., MIN6, glucose diffusion, Jordan

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