Current HPLC Methods for Determination of Medicaments in Formulations and Biological Samples

Mitsuhiro Wada, Suleiman M. Alkhalil, Kenichiro Nakashima

Abstract


The performance of high-Performance liquid chromatography (HPLC) instruments has been remarkably progressed. As a result, an HPLC method plays a conspicuous role in analysis of medicaments in formulations and biological samples. The varied detection methods used for HPLC such as ultra violet (UV), mass spectrometry (MS), fluorescence (FL) and chemiluminescence (CL) detections in addition to electrochemical detection (ECD) with suitable pretreatment or labeling were chosen in response to the purpose of analysis. Analysis of medicaments in formulations was mainly performed by UV detection, meanwhile EC, MS, FL and CL detections with high sensitivity were used for analysis of medicaments in biological samples. The sensitivity ranging from microgram to picogram level could be achieved. In this review, current HPLC methods for determination of medicaments in formulations and biological samples were described. Furthermore, their advanced applications for chiral analysis and pharmacokinetic drug-drug interaction evaluation of medicaments were presented.

Keywords


HPLC, Medicaments, Formulations and Biological Samples

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