Phytochemical Analysis and Anti-inflammatory Activity of Various Extracts Obtained from Floral Spikes of PRUNELLA VULGARIS L.

Gazanfar Ahmad, M. H. Masoodi, Nahida Tabassum, Rashid Ahmad Mir

Abstract


Prunella vulgaris L. also known as self-heal has been extensively used for treating various ailments in traditional system of medicine which indicates that the plant is an important source of various bioactive constituents. Current study was performed to carry out qualitative and quantitative phytochemical evaluation and anti-inflammatory activity of various extracts obtained from floral spikes of P. vulgaris. Different extracts were obtained from shade dried floral spikes of P. vulgaris using cold percolation method. The extracts were subjected to suitable phytochemical tests for the detection and estimation of various phytoconstituents. Aqueous and methanolic extracts were subjected to inflammatory activity in animal models using carrageenan paw edema method at a dose of 300 mg/kg of body weight. Diclofenac (10 mg/kg) was used as reference standard. Phytochemical analyses revealed the presence of flavonoids, tannins, saponins, carbohydrates, steroids, alkaloids and anthraquinone glycosides. The exact percentage of phytoconstituents obtained by quantitative estimation for alkaloids, saponins, tannins and carbohydrates was 0.255±0.001, 2.676±0.0141, 0.0988±0.005, 1.584±0.09 per 100 g of plant material, respectively. Both aqueous and methanolic extracts showed anti-inflammatory activity with maximum percentage inhibition of inflammation of 28.965 and 30.860, respectively while maximum percentage inhibition of inflammation shown by Diclofenac was 60.075. Phytochemical analyses revealed the presence various phytoconstituents which justify its role for the treatment of various ailments in folklore system of medicine. Besides it can be concluded that both aqueous and methanolic extracts show significant (P<0.05) anti-inflammatory activity comparable to reference standard diclofenac (P<0.01) when compared with control group.

Keywords


Prunell vulgaris, Phytoconstituents, Anti inflammatory, extracts

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