Production and optimization of cellulolytic enzymes from Trichoderma isolates under solid state fermentation

weldesemayat gorems woldemariam

Abstract


Agricultural wastes were used as sole carbon sources for the production of cellulases by Trichoderma isolates in solid state fermentation. Carboxymethyl cellulose and Congo Red were used to screen four isolates that had stronger ability to produce cellulase enzymes for further study. Cellulase production was assayed by measuring the amount of glucose liberated in μmol/ml/min by using the DNS assay method at 540 nm. Maximum cellulase enzyme was recorded between 5-11 days incubation. Wheat bran, Rice bran and wheat straw were found suitable for high cellulase production whereas cotton seed, coffee pulp and barely bran relatively showed the least cellulase production in SSF. Trichoderma isolates, AUT1 produce the highest carboxymethyl cellulose on wheat straw (5.68 U/g), AUT5 on rice bran (8.15 U/g), AUT2 and AUT4 on wheat bran, their enzymatic activities were 4.92 U/g and 7.01 U/g, respectively. However, the isolate AUT5 gave the highest carboxymethyl cellulose (8.15 U/g) on rice bran whereas isolate AUT2 produce the least this enzyme on cotton seed (1.02 U/g). The maximum amount of cellulase was observed between 55% to 65% moisture contents. It is evident from the present study that solid state fermentation was better for the production of maximum cellulase from Trichoderma isolates using agricultural wastes.

Keywords


Agricultural wastes, cellulase, production and optimization, solid state fermentation and Trichoderma isolates

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