Development of an efficient screening technique to assess the genetic variability to response of some Chickpea varieties (Cicer arietinum L.) to water stress

yanal ahmad alkuddsi

Abstract


This study was conducted to develop a method of screening technique allows quickly and effectively to detect genetic variability in bearing varieties of chickpea for osmotic stress tolerance ,in addition to assessing the value of improving the ability of incitement in the chickpea plant with lethal levels of osmotic stress. So this study was carried out in the laboratories of Faculty of Agriculture ,Department of Agronomy at the University of Damascus , with the aim of exploring the genetic variability in the response of some genetic genotypes of chickpea to osmotic stress (water stress induction 6000-PEG) adoption of laboratory screening method. The results of the study showed that the treatment -0.2 Mpa was the optimum induction of osmotic stress, while the treatment -1.9 Mpa was the optimum lethal level of osmotic stress. The results showed significant differences in the effect of different lethal levels of osmotic stress in length of roots and seedlings. The proportion of the decrease in length of roots and seedlings with increased severity of osmotic stress and the treatment which consider as an optimum lethal level caused decreasing in roots and seedling length 50% compared with the control at the end of the period of recovery growth. Significant differences also observed in the effect of different induced levels on the growth and development of chickpea’s roots and seedlings. The treatment -0.2 Mpa consider as the optimum induction level, because the reduction in the length of roots and seedlings at this treatment was less, but this treatment has caused an increase in the growth of the induced seedlings and the growth of this seedling was more than the growth of non- induced seedling (control) at the end of the period of recovery growth. During the evaluation of genetic variability in bearing varieties of chickpea to osmotic stress in the seedling stage, significant differences were observed in the response of chickpea varieties to osmotic stress and the variety Gab 4 categorized as genotype resistant to osmotic stress because the reduction in the length of the roots (39.92%), and seedlings (24.46%) was lower compared with the other varieties, while the evaluation of Gab 3 and Al- baladi chickpea as a sensitive genotype to osmotic stress because the reduction in the length of the roots (48.10% & 49.85% respectively) and the seedlings (34.38% and 39.03% respectively) were higher in comparison with the other varieties, and the variety Gab 5 categorized as medium to sensitive tolerance to osmotic stress. This refers to real genetic variability among varieties in response to osmotic stress, even though expose all the varieties to the same level of induction. The roots of the chickpea more sensitive to osmotic stress of the aerial parts.

Keywords


optimum lethal level, optimum induction level, genetic variability, osmotic stress, screening technique, chickpea.

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