Influence of Some Carbohydrates on in vitro Regeneration of Albizzia (Albizzia julibrissin Durazz.) on Growth Regulator-Free Media

Hussein Al-Zu'bi

Abstract


The effect of three carbohydrates; i.e. sucrose, fructose, xylose or a mixture of fructose and xylose on in vitro regeneration of Albizzia julibrissin using hypocotyl explants was investigated. Carbon source had considerable effect on the different morphogenic responses, mainly on callus, shoot and root induction. It was shown that the regeneration of whole rooted plants on growth regulator-free media using xylose as a sole carbon source could be achieved. Xylose gave better results in the induction of shoots and also roots, in comparison with sucrose which is commonly used in tissue culture media. The results of the present work open numerous perspectives concerning both fundamental and applied approaches for better understanding of morphogenesis in relation with tissue nutrition through manipulation of the carbon source used. Furthermore, the method used in the regeneration on growth regulator-free media may ensure true-to-type multiplication and avoid abnormalities which might be caused by growth regulators added to culture media.

Keywords


Albizzia julibrissin, Regeneration ,Auxin, Morphogenesis, Carbohydrates, In vitro

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