Studies of Factors Affecting Rooting of GF 677 Rootstock by Plant Tissue Culture Technique

W Mohsen, A Dakah, S Zaid


This research studiedof effects of in vitro rooting of GF 677 rootstock by plant tissue culture technique. Single cuttings 0.5 – 1 cm in length withone bud excised and cultured on woody plant medium (WPM) supplemented with 6.6 µM benzyl amino purine(BA) + 1µM indole-3-butyric acid IBA which showed the best result for proliferation (4.58). Microshoots (1-2 cm) moved to rooting media containing different concentrations of IBA for studying the effect of incubation of shoots in dark, kind of the carbon source, macro-elements concentrations and active charcoal onrooting rate, number and length of the formed roots. Maximum rooting rate was obtained on WPM + 9.84µM IBA(90%) when the shoots incubated in the dark for the first 7 days after the cultured on the medium, the highest number of the root was 3.94 with maximum length of 4.5 cm. Reducing macro-elements concentration led to reduce the rooting rate and number of root from 3.94 to 3.69 and length from 4.57 to 4.36 cm, and so for the addition of active charcoal that reduce the rooting rate and number of root from 3.94 to 3.92 but length of root increase from 4.57 to 4.59 cm. On the other hand, rooting rate did not change significantly when the carbon source was changed and the number of roots was 3.94 in the medium supplemented with sucrose and 3.6 in the medium supplemented with sorbitol, length of roots was 4.57 cm with sucrose and 4.6 cm with sorbitol. Rooted microshoots were successfully acclimatized (95%) on pots mixture of 2:1 (v/v) peat/perlite after one month.


Rooting, tissue culture, GF 677 rootstock.

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