In vitro Propagation of Date Palm (Phoenix dactylifera L.) cv. Khaddrawi by Direct Organogenesis

Ahmed Madi Waheed AL-Mayahi

Abstract


The present invistigation was conducted to study direct shoot initiation on date palm shoot (cv. Alkhaddrawi) from different explants source .The rsuults showed that different explants cultured on MS medium supplemented with Indol-3-butyricacid (IBA) and benzyladenine(BA) at 4.0 and 1.0 mg/L, respectively stimulated the growth of ex-plant and their development of combination 4.0 mg/L 2iP + 2.0mg/L BA + 0.5 mg/LNAA) stimulated the emergence and proliferation of axillary buds.Micoshoots (2.5 cm) were rooted on medium supplemented with naphthalene acetic acid "NAA " at 0.1, 0.5 or 1.0 mg / L. Moreover, rooting formation increased up to70-80% with the using of NAA. On other hand, medium supplemented with 0.1 mg/L NAA resulted the best root quality. Acclimatization increase percentage when rooting ex-plant transferred into soil pots composed of perlit and peat moss 1:2.

Keywords


Tissue Culture, Axillary Buds, Date Palm, Growth Regulators

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