Response of Pilea Plant Pilea cadieri for Micropropagation using Tissue Culture Techniques

Laurene Layous

Abstract


An efficient micropropgation of Pilea cadieri using tissue culture techniques were developed. The shoot cultures were cultured on Murashige and Skoog media supplemented with 30 g/L sucrose, 7g/L agar – agar, and different concentration of benzyl amino purine (BAP) (0, 0.5, 1 , 1.5 mg/L), and Naphthalene Acetic Acid (NAA) (0 , 0.5 mg/L). While reducing mineral to half with 20 g/L sucrose and addition of indole-3-butyric acid (IBA) at different concentrations (0, 0.5, 1, 1.5 mgl-1). Maximum open buds was obtained with BAP only in the media at the phase of initial culture, concentration (1.5 mg/L) gave the highest values of open buds (100%) and maximum number of shoot per initial explant (3.3). Also secondary multiplication for a greater number of shoots (6.15 shoot/explant), thereby increasing the overall propagation rate (9.45 shoot/explant). The in vitro propagated shoots produced roots when transferred to MS medium containing indole-3-butyric acid (IBA) at various concentrations, at 1.0 mg/L (IBA) 100% shoot were successfully rooted after one month of culture. Rooted micro shoots were potted and hardened. 79.62% microshoots were successfully rooted after three months. The profitability final per explants is 7.52 viable plants successfully in ambient conditions after 8 months of primary culture and after taking into account both the rate of rooting and the success rate of hardening.

Keywords


Pilea Plant, In vitro, Micropropagation, Rooting, Growth Regulators.

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