Isolation and Characterization of Streptococcus bovis from Raw Cow Syrian Milk

A. E. Abou Younes

Abstract


This research aims to identify Streptococcus bovis bacteria isolated from Row Syrian milk. The samples were collected from farms located in the cities and the suburbs of Damascus, , Dara'a and Alqunitera. Moreover, specimen were isolated from 123 milk samples, of which 320 specimen were coccus, gram positive by growing on M17 media at 37ºC. Nevertheless, these samples were assembled during the period of June 2009 till May 2011. Both PCR and API 20 Strep Techniques were used for identifying these bacteria. The study also showed that 98 samples grown on M17 media, belongs to Strep. bovis bacteria at (30,63%). Moreover, the study found out that three subordinates under this type of bacteria where the most spreading ones, and they were II1 with 45,91%, then II2 with 32,65% and finally the percentage of the third I was 21,44%. According to this study, it appeared that the Strep. Bovis bacteria exists seasonally and it is mainly in both summer and spring. Which is due to the kind of nutrition used in feeding the cows. The green used feed may have high load of bacterial that can be transferred hence to the animals. Finally, confirming results were diagnosed using both API 20 Strep and PCR technique equally.

Keywords


Streptococcus bovis , Classification, Isolation, Row Cow Syrian Milk, PCR, API 20Strep

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