Molecular Characterization of Two (SLCV) Isolates: One from the Jordan Valley and the Other from Southern Syria

Nasir Al-Tamimi, H. Kawas, A. Mansour, A. Arabiat, Nida Salem

Abstract


This study was conducted during the growing seasons 2005/2006 and 2006/2007 in order to distinguish by molecular methods between 2 isolates of (SLCV) isolated one from Southern Syria and the other from the Jordan Valley referred to hereafter as SYR-C2 and JOR-C1 for Syrian and Jordanian isolates, respectively. The study provides the first report of SLCV in Syria and extends information about the relatedness with the Jordanian isolate and other isolates in the region. The (SLCV) virus appeared in recent years in the two countries as a serious disease, causing big losses of squash crops, especially in the Jordan Valley. PCR amplifications were performed by using a pair of oligonucleotide primers (SLCV-1 and SLCV-2) specific for coat protein region. The respective band was about 1100bp in size. Results of DNA sequencing showed a similar arrangement of DNA nucleotides of the Syrian and Jordanian isolates in about 95%, as well as a similar arrangement with the Egyptian isolate Cairo (Accession no.DQ285019.1) in about 99% and 95%, respectively and with the Jordanian M (Accession no. EF532620) isolate in about 98% and 94%, respectively. The virus (SLCV) is transmitted by the white fly (Bemisia tabaci) and is one of the important viruses detected in the region causing significant economic losses, especially when plants contracted the disease at an early stage of their growth

Keywords


SLCV isolates, Southern Syria, Jordan Valley, Squash viruses

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