Studying the Genetic Relationship of Some Cultivated Wild Olive Types in Mesiaf Region by Using ISSR Technique

Ghada Kattmah, Faisel Hamed, Souhel Makhool

Abstract


A fingerprint experiment was conducted on eight phenotypes of cultivated wild olive in Mesief region – Hamah Province, Syria in order to study the genetic distance among these types, by using 27 primers of ISSR technique.
ISSR technique was able to discriminate among the wild types included in the study, the used primers have given 1065 bands 689 of which were polymorphisms and the general average has reached 25.5 polymorphism/ primer, whereas the total band lines were 281 and 211 of them were polymorphisms with an average of 7.8 polymorphism lines / primer.
The results have revealed high genetic variation among studied types. The values of dissimilarity ranged between 17 and 43%. The lowest value of dissimilarity has been found between WH10 and WH11; whereas the highest one was between WH16 and WS30.
The studied types were clustered according to geographic diffusion in the fields; whereas these clustering groups of types have not coincided with the height of their region above sea level, and the ISSR marker technique was useful in identifying most of these types by a unique DNA band.

Keywords


Cultivated wild olives, Genetic relation, ISSR

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