Cryopreservation by Encapsulation-vitrification of Embryogenic Callus of Wild Crocus (Crocus hyemalis and Crocus moabiticus) (Research Note)

S. H. Baghdadi, R. A. Shibli, M. Q. Syouf, M. A. Shatanawi, A. Arabiat, I. M. Makhadmeh

Abstract


Crocus is asexually propagated plant by corms and it is susceptible to a wide range of pathogens and environmental stresses. It cannot be effectively stored for long periods using conventional methods. Cryopreservation by encapsulation-vitrification was studied as a long-term conservation method. Callus fragments were encapsulated in 4 mm calcium alginate beads containing hormone-free MS medium. Encapsulated beads were dehydrated with the Plant Vitrification Solution 2 (PVS2) [(w/v) 30% glycerol, 15% DMSO and 15% EG] for 10, 20, 30, 60 and 90 minutes and dipped in liquid nitrogen for at least 1 h. Dehydration of cryopreserved encapsulated callus fragments with PVS2 for 20 minutes resulted in 100% survival and regrowth prior to cryopreservation for both species. After cryopreservation, the highest survival (55.6% and 75.0% for C. moabiticus and C. hyemalis, respectively) and highest regrowth (66.7% for both species) were obtained for 20 minutes dehydration with PVS2.

Keywords


Crocus hymalis, Crocus moabiticus, Encapsulation, Verification, PVS2

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