Variation in Hyoscyamus spp. Populations from Jordan Using Morphological Traits and RAPD Markers

Samir M. Abou-Isba, Adel H. Abdel-Ghani, Saleh Al-Qura’n

Abstract


Variation of twelve Hyoscyamus aureus, four H. desertorum and two H. reticulatus populations from Jordan
were investigated at the phenotypic level with morphological characters and at DNA level using the Random
Amplified Polymorphic DNA (RAPD) technique. Sixteen morphological traits were measured after 100 days
from planting. There was a significant difference among the collected populations in 12 out of 16 morphological
traits. The three highly polymorphic selected primers generated 60 RAPD bands with 39.5% mean level of the
proportion of polymorphic loci. Multivariate procedures using dendograms and Principle Coordinate Analysis
(PCoA) were used to classify the collected populations on the basis of phenotypic traits and RAPD fragments.
RAPD estimate of genetic relationships among Hyoscyamus species was consistent with morphological study
results. From the cluster analyses using dendograms and PCoA, it appears that morphological traits and RAPD
markers were useful to separate the populations at the species level. Cluster analysis of the morphological and
RAPD data indicates that H. aureus and H. desertorum populations were grouped with each other, revealing
closer genetic relationships of the two species with each other than with H. reticulatus. A wide range of genetic
distances was observed among populations within the same species, which might be due to high degree of
ecogeographical heterogeneity among collection sites. In conclusion, the present study serves as a base
knowledge of the phylogenetic relationships for future studies among and within the genus Hyoscyamus.

Keywords


Hyoscyamus spp., Genetic Variation, Morphological Characters, Phylogenetic Relationship,

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