Vitrification and Droplet-Vitrification Cryopreservation for Wild Artemisia (Artemisia herba-alba Asso.) Shoot-tips

Sarab A. Sharaf, Rida A. Shibli, Mahmood A. Kasrawi, Tamara S. Al-Qudah

Abstract


This study shows the effect of conserving shoot-tips of Artemisia herba-alba via two vitrification based cryopreservation techniques (Vitrification and droplet-vitrification). In vitrification three separate experiments were conducted to study the effect of loading and cryoprotectant solutions and their concentrations on the survival and regrowth of Artemisia shoot-tips, The simplest efficient cryopreservation vitrification dependent technique of Artemisia herba-alba shoot tips was by using two-step increased concentration of PVS2 as it gave high survival (89%) and regrowth (81%) percentages after freezing. In droplet vitrification the shoot-tips were vitrified with chilled plant vitrification solution and placed on aluminum foil strips prior to plunge in liquid nitrogen. Droplet-vitrification technique was suited method for conserving Artemisia herba-alba shoot tips giving high survival (93.3%) and regrowth (73.3%) after 35 min of exposure to PSV2 and after plunge in liquid nitrogen.

Keywords


Artemisia, cryopreservation, droplet, preculture, shoot tips, vitrification

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