First Detection of Honey Bee Viruses in Jordan by RT-PCR

Nizar Haddad, Mohammad Brake, Hussein Migdadi, Joachim R. de Miranda


Mortality of honeybees is a serious problem that beekeepers have to face periodically in Jordan and worldwide. The presence of honeybee viruses, in addition to other pathogens and parasites may be one of its possible causes. In this work, individual bee samples from Ajlon area of Jordan were screened for six honey bee viruses using RT-PCR and specific primers. Acute Bee Paralysis Virus (ABPV), Sac Brood Virus (SBV) and Deformed Wing Virus (DWV) were detected in the samples, while Black Queen Cell Virus (BQCV), Chronic Bee Paralysis Virus (CBPV) and Kashmir Bee Virus (KBV) were not detected. This is the first report of the detection of bee viruses in Jordan utilizing molecular techniques.


Honeybee viruses, RT-PCR, Molecular markers

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