Determination of Benzene and its Metabolite Phenol in the Urine Samples of Gas-Station Workers in the Greater Amman Municipality

Mahmoud A. Alawi


Thirty one urine samples were gathered from workers in twenty automobile gas- stations in the area of Greater Amman Municipality. The samples were analyzed for the contents of the aromatic benzene and its main metabolite phenol using High Performance Liquid Chromatography (HPLC) with the UV-detector after steam distillation and Liquid-liquid extraction. The benzene concentration in the urine samples ranged from 0.80 to 9.44 mg/L and for phenol between 0.16 and 20.77 mg/L. A clear correlation was found between the concentrations of phenol and benzene in the urine samples. Workers whom urinary benzene concentration was 6.5 mg/L or higher were found after six years that they get the Leukemia.


Benzene, Phenol, Gas- Station Workers, Urine, Amman.

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