Immune Effects of Partially Purified Polyamine Oxidase(PAO) from Cerebrospinal Fluid(CSF) Against Infection with Secondary Hydatid Disease in Mice.I. Phagocytosis and Delayed-Type Hypersensitivity

Khawla A. Al-Flayeh, Asmaa Abdulaziz, Wathba I. Ali

Abstract


The study investigated the immune system response to infection with secondary hydatid disease in BALB/c mice activated by PAO with spermine then infected with protoscoleces of Echinococcus granulosus. Pathological changes occurred in mice activated by PAO-Spm , and by Spm alone, were followed in comparison with the positive control group throughout one month, depending on certain criteria which included changes in non-specific and specific immune response represented by phagocytosis and delayed–type hypersensitivity, respectively.The results revealed that PAO-Spm system is better than Spm alone ,and the optimum concentration of PAO-Spm is 1200 µg PAO with 200µg Spm.

Pathological changes were followed in activated mice with the optimum concentration of PAO in comparison with +ve control group throughout two and three months. The results revealed an increase in the rate of phagocytic index and foot pad thickness, respectively, in activated mice with PAO-Spm, Whereas an increase in the phagocytic index, but no change in foot pad thickness occurred in mice activated with Spm alone.

Therefore, it was concluded that PAO isolated from CSF with Spm could be considered as an effective immunumodulator against infection with secondary hydatid disease.


Keywords


Polyamine Oxidase, Hydatid Disease, Immunity.

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