Investigation of the Effect of Some Ultrasonic Waves and Electrotreatment in Callus Initiation and Plant Regeneration of Ricinus Communis L. Seedlings

Shifaa Mahdi Salih, Rasha Fawzi Al-Obaedi

Abstract


Explants of different parts (shoot tips, leaves, stems, cotyledonary leaves, hypocotyls and roots) of Ricinus communis seedlingsin addition to the callus derived from these explants, were exposed to ultrasonic waves at a frequency of 47.6KHz for 5,10, 15, 20, 30, 40, and 60 minutes. For the first time. The explants were cultured on agar solidified Murashige and Skoog medium containing various concentrations of Naphthalen acetic acid (NAA) and Benzyl adenine (BA).The electrotreatment involves the exposure of the above explants and its callus to different voltages (200, 500, 1000 volt) for 1, 5,10 milli second. The Results indicated the encouraging effect of ultrasonic waves in callus initiation and its growth. In fact, the inductive effect was represented by reducing the time needed for callus induction and the fresh weight of callus. Also, the protein content was increased in most samples. In addition, the ultrasonic waves had a stimulating effect on increasing the ability of shoot tips callus for shoots regeneration to reach 77.77% when exposed for 60 minutes. This study showed, in one aspect, the efficient and stimulative effect of electrotreatment in callus initiation, growth, protein content and differentiation of R. communis. The positive effect of this technique is reflected on the ability of stems and shoot tips callus for shoot formation as the percentage reached 120 % and 90 % when callus exposed to 200 and 1000 volt for 10msec, respectively.

Keywords


Ricinus communis, Ultrasonic Waves,Callus, Electrotreatment.

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