Genetic Diversity Analysis of Achillea fragrantissima (Forskal) Schultz Bip. Populations from Jordan Using Amplified Fragment Length Polymorphism (AFLP)

Ibrahim M. Rawashdeh, Nasri I. Haddad, Ahmed Amri


Amplified fragment length polymorphism (AFLP) analysis was used to study the genetic variation among and within populations of Achillea frgarntissima (Forskal) Schultz Bip. Species collected from five regions in Jordan. Five primer combinations were used which produced about 3297 amplified fragments, out of which 1137 are polymorphic, showing high genetic variability among populations (regions) and within populations. The dendrogram culster and similarity analysis obtained by the Unweighted Pair-Group Method with arithmetic average (UPGMA) allowed the discrimination of individual entries into eleven clusters with accessions from the same region most often included in the same cluster. For example, the eighteen entries of Shoubak populations formed a separated group. High similarity (48%) among all tested entries was observed between the two samples of Shoubak 3 (26 and 27) and the two samples of Mwaqqar 2 (76 and 81). AFLP is confirmed as a powerful tool to detect genetic diversity among and within collected populations of A. frangrantissima, which will guide future collection and conservation efforts of this species.

الكلمات المفتاحية

Achillea fragrantissima, Polymorphism, Genetic Diversity, Jordan.

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