Detecting Genetic Diversity among Wild Species, Landraces, Mutants, and Cultivated Chickpea Using RAPD Analysis (Research Note)

Ibrahim M. Rawashdeh, Nasri I. Haddad


Collection, conservation and use of chickpea genetic resources are necessary for agricultural development. The efficiency of these activities will depend on the ability to discriminate and to detect the genetic relationships among germplasm. Twenty-three primers of Random Amplified Polymorphic DNA (RAPD) markers were used to examine the genetic diversity among different wild and cultivated chickpea genotypes. The degree of polymorphism was detected by using only three primers: OPA07, OPA20 and OPZ20. Accession-specific RAPD markers and unique band patterns were also found. A dendrogram based on Unweighted Pair Group Method cluster Analysis (UPGMA) was constructed using a similarity matrix derived from the RAPD amplification products generated by using these primers. The results of the DNA analysis showed a considerable variability among Jordanian chickpeas landraces and wild species, which are also confirmed by agronomic and morphologic data. This variability should be conserved, evaluated further, and utilized in the improvement of the chickpea crop.

الكلمات المفتاحية

Cicer, Genetic Polymorphism, PCR DNA Amplification, RAPD.

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